ELISA Mouse Peripheral myelin protein 22 (PMP22)

Reactivity:Mouse (Mus muscµLus) UniProt:P16646 Abbreviation:PMP22 Alternative Names:CMT1A; CMT1E; DSS; GAS-3; HMSNIA; HNPP; MGC20769; Sp110; growth arrest-specific 3 Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.65 ng/mL Intra-AssayCV:?5.7% Inter-AssayCV:?8.9% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PMP22 in samples. An antibody specific for PMP22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMP22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PMP22 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMP22 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Peripheral myelin protein 22, also known as PMP22, is a protein encoded by the PMP22 gene. Alternative splicing of this gene resµLts in three transcript variants that encode the same protein. The integral membrane protein encoded by this gene is a hydrophobic, tetraspan glycoprotein expressed mainly in Schwann cells and is a major component of compact myelin in the peripheral nervous system.Various mutations of the gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and Hereditary neuropathy with liability to pressure palsies (HNPP). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).