ELISA Mouse Polyamine-modulated factor 1-binding protein 1 (PMFBP1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9WVQ0 Abbreviation:PMFBP1 Alternative Names:DKFZp434G131; FLJ40146; PMF-1 binding protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.061 ng/mL Intra-AssayCV:?6.1% Inter-AssayCV:?8.1% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PMFBP1 in samples. An antibody specific for PMFBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMFBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PMFBP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMFBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:May play a role in sperm morphology especially the sperm tail and consequently affect fertility. May also be involved in the general organization of cellµLar cytoskeleton. Cotransfection of PMF1 and NRF2 activated transcription from the polyamine-responsive element of the SSAT promoter in a reporter assay, and PMF1 was the rate-limiting component.Mutations that interrupted either of these regions altered the ability of the proteins to interact, and they lost their ability to regµLate transcription of the SSAT gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).