ELISA Mouse Phosphoglycerate mutase 1 (PGAM1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9DBJ1 Abbreviation:PGAM1 Alternative Names:RP11-452K12.8; PGAM-B; PGAMA; BPG-dependent PGAM 1|phosphoglycerate mutase 1|phosphoglycerate mutase A; nonmuscle form Application:ELISA Range:25-1600 pg/mL Sensitivity:6.25 pg/mL Intra-AssayCV:?5.1% Inter-AssayCV:?8.6% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PGAM1 in samples. An antibody specific for PGAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PGAM1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Phosphoglyceric acid mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The fact that the PGAMA and GOT1 loci are linked in the mouse supports the assignment of PGAMA to chromosome 10. PGAM is a dimeric enzyme containing, in different tissues, different proportions of a muscle (MM) isozyme, a brain (BB) isozyme, and a hybrid form (MB). Electrophoresis of normal adµLt muscle PGAM shows marked predominance of the MM band with only faint BB and MB bands. In most other tissues, including brain, liver, erythrocytes, and leukocytes, PGAM-BB is the only demonstrable isozyme. In cardiac muscle extracts, all 3 bands are seen, althoµgh PGAM-MM predominates. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).