ELISA Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q91ZX7 Abbreviation:LRP1 Alternative Names:A2MR; APOER; APR; CD91; FLJ16451; IGFBP3R; LRP; MGC88725; TGFBR5; TbetaR-V/LRP-1/IGFBP-3 receptor|alpha-2-macroglobµLin receptor|low density lipoprotein-related protein 1|type V tgf-beta receptor Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.63 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.7% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LRP1 in samples. An antibody specific for LRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LRP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Low density lipoprotein receptor-related protein 1 (alpha-2-macroglobµLin receptor), also known as LRP1 or CD91, is a protein forming a receptor found in the plasma membrane of cells involved in receptor-mediated endocytosis.Neurons require cholesterol to function. Cholesterol is imported into the neuron by the apolipoprotein apoE4 via LRP1 receptors on the cell surface. It is thoµght that a causal factor in Alzheimer's is the malfunction of this process which damages neurons by starving them of cholesterol.The protein showed strong calcium binding. Kristensen et al. (1990) and Strickland et al. (1990) demonstrated that LRP is identical to the alpha-2-macroglobµLin (A2M) receptor (A2MR). Like the mannose-6-phosphate receptor, the A2MR/LRP molecµLe is probably bifunctional. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).