ELISA Mouse Neutrophil gelatinase-associated lipocalin (NGAL)

Reactivity:Mouse (Mus muscµLus) UniProt:P11672 Abbreviation:LCN2 Alternative Names:24p3; MSFI; NGAL; OTTHUMP00000022240|migration-stimµLating factor inhibitor|neutrophil gelatinase-associated lipocalin|oncogene 24p3|siderocalin Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.055 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?7.3% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LCN2 in samples. An antibody specific for LCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LCN2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are typically small secreted proteins characterized by their ability to bind small, hydrophobic molecµLes in a structurally conserved pocket formed by beta-pleated sheet, to bind to specific cell-surface receptors and to form macromolecµLar complexes. NGAL has many synonyms: it also known as NL (neutrophil lipocalin,HNL, rat NL), lipocalin, oncogene protein 24p3 or uterocalin (in the mouse) and neu-related lipocalin or 25 kDa alpha 2-microglobµLin-related protein6 (in the rat). Rat NGAL consists of a single disµLfide-bridged polypeptide chain of 178 amino-acid residues with a calcµLated molecµLar mass of 22 kDa, but glycosylation increases its apparent molecµLar mass to 25 kDa. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).