ELISA Mouse Deoxyribonuclease gamma (DNASE1L3)

Reactivity:Mouse (Mus muscµLus) UniProt:O55070 Abbreviation:DNASE1L3 Alternative Names:DHP2; DNAS1L3; LSD; DNase I homolog protein 2|DNase gamma|LS-DNase|Liver and spleen DNase|deoxyribonuclease I-like III|deoxyribonuclease gamma Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.078 ng/mL Intra-AssayCV:?5.7% Inter-AssayCV:?10.4% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DNASE1L3 in samples. An antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DNASE1L3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DNASE1L3 encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroµghly characterized. The predicted 306-amino acid protein was 45% identical to that of DNASE1. Northern blot analysis showed that DNASE1L3 was expressed primarily in spleen and liver cells. The predominant mRNA was 1.3 kb, althoµgh there were 2 other transcripts. The purified recombinant protein had DNase activity in vitro that, unlike that of DNASE1, was not inhibited by G-actin. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).