ELISA Mouse Doublesex- and mab-3-related transcription factor A1 (DMRTA1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8CFG4 Abbreviation:DMRTA1 Alternative Names:DMO; MGC163307; MGC163309; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.3% Inter-AssayCV:?8.4% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRTA1 in samples. An antibody specific for DMRTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DMRTA1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DMRTA1, Belongs to the DMRT family. Contains 1 DM DNA-binding domain. Expressed in liver, kidney, pancreas, prostate and weakly detected in testis and ovary.May be involved in sexual development. The Dmrt genes encode a large family of transcription factors whose function in sexual development has been well studied in invertebrates and vertebrates. Their expression pattern is not restricted to the developing gonads, indicating that Dmrt genes might regµLate other developmental processes. By genomic analysis of zebrafish and rat DMRT genes, all protein sequences of the vertebrate DMRTs were searched from gene databases and aligned. Phylogenetic tree of all these DMRT genes was reconstructed and evaluated by Bootstrap method. These DMRT genes were clustered into seven subfamilies. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).