ELISA Mouse Delta-like protein 4 (DLL4)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9JI71 Abbreviation:DLL4 Alternative Names:MGC126344; hdelta2; delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|notch ligand DLL4|notch ligand delta-2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.1% Inter-AssayCV:?10.4% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLL4 in samples. An antibody specific for DLL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLL4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DLL4 is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. The predicted 685-amino acid DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands. It has an extracellµLar region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs. DLL4 shares 87% amino acid sequence identity with mouse Dll4. Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).