ELISA Mouse Disks large-associated protein 2 (DLGAP2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8BJ42 Abbreviation:DLGAP2 Alternative Names:DAP2; SAPAP2; PSD-95/SAP90-binding protein 2|SAP90/PSD-95-associated protein 2|discs large-associated protein 2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.9% Inter-AssayCV:?8.7% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLGAP2 in samples. An antibody specific for DLGAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLGAP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The product of this gene is one of the membrane-associated guanylate kinases localized at postsynaptic density in neuronal cells. These kinases are a family of signaling molecµLes expressed at various submembrane domains and contain the PDZ, SH3 and the guanylate kinase domains. This protein may play a role in the molecµLar organization of synapses and in neuronal cell signaling. Alternatively spliced transcript variants encoding different isoforms have been identified, but their fµLl-length nature is not known.Sequence analysis predicted that the 538-amino acid DLGAP2 shares 52% sequence similarity with DLGAP1 and contains four 14-amino acid repeats and 3 proline-rich regions. Binding analysis showed that DLGAP2, like DLGAP1, interacts with DLG4 and DLG1. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).