ELISA Mouse Dickkopf-related protein 4 (DKK4)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8VEJ3 Abbreviation:DKK4 Alternative Names:DKK-4; MGC129562; MGC129563; dickkopf homolog 4 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.051 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?10.2% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DKK4 in samples. An antibody specific for DKK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDKK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DKK4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. Western blot analysis showed that DKK4 is secreted as 40-, 30- to 32-, and 15- to 17-kD proteins; these proteins were not significantly affected by N-glycanase treatment. N-terminal sequence analysis of the different forms sµggested that DKK4 is proteolytically processed, possibly by intracellµLar furin-type proteases. Functional analysis determined that DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).