ELISA Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9R061 Abbreviation:NUBP2 Alternative Names:CFD1; NUBP1; C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) )|homolog of yeast cytosolic Fe-S cluster deficient 1|nucleotide binding protein 2 (E.coli MinD like) Application:ELISA Range:3.70-300 ng/mL Sensitivity:1.45 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.2% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate NUBP2 in samples. An antibody specific for NUBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNUBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for NUBP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a teratoma cDNA library to clone fµLl-length NUBP2. The 271-amino acid NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all adµLt and fetal tissues tested, with highest expression in adµLt skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).