ELISA EP300 anti-

Quantity :50µg Clone Number: Aliases:E1A associated protein p300 antibody; E1A binding protein p300 antibody; E1A-associated protein p300 antibody; EP300 antibody; EP300: E1A binding protein p300 antibody; EP300_ antibody; Histone acetyltransferase p300 antibody; KAT3B antibody; p300 HAT antibody; RSTS2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q09472 Immunogen:Recombinant Histone acetyltransferase p300 protein (2283-2414AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 Background:Functions as histone acetyltransferase and regµLates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regµLation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at \\\'Lys-122\\\' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimµLates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at \\\'Lys-27\\\' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates \\\'Lys-131\\\' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 throµgh acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modµLation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. RegµLates Tat\\\'s transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regµLated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at \\\'Lys-626\\\' which is essential for its transcriptional coactivator activity (PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:EP300 Research Areas:Epigenetics and Nuclear Signaling; Cancer; Metabolism; Stem cells