ELISA Phosphatidate phosphatase LPIN1 (LPIN1)

Reactivity: (Homo sapiens) UniProt:Q14693 Abbreviation:LPIN1 Alternative Names:DKFZp781P1796; KIAA0188; PAP1; Application:ELISA Range:6.25-100 ng/mL Sensitivity:2.43 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?7.8% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LPIN1 in samples. An antibody specific for LPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LPIN1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Lipin-1 has phosphatidate phosphatase activity.This gene represents a candidate gene for lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insµLin resistance. Mouse studies sµggest that this gene functions during normal adipose tissue development and may also play a role in triglyceride metabolism. Database analysis identified the LPIN1 gene, as well as the and mouse LPIN2 and LPIN3 genes. Consistent with the observed reduction of adipose tissue mass in fld mice, wildtype Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of 3T3-L2 preadipocytes. The resµLts indicated that lipin is required for normal adipose tissue development, and provided a candidate gene for lipodystrophy. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).