ELISA 12-hydroxyeicosatetraenoic acid (12-HETE)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:12-HETE Alternative Names:N/A Application:ELISA Range:12.35-1000 ng/mL Sensitivity:4.64 ng/mL Intra-AssayCV:?4.1% Inter-AssayCV:?7.6% Recovery:0.94 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate 12-HETE in samples. An antibody specific for 12-HETE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any12-HETE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for 12-HETE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 12-HETE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:12-Hydroxyeicosatetraenoic acid (12-HETE) is an eicosanoid. It is a metabolite of arachidonic acid produced by the action of the enzyme arachidonate 12-lipoxygenase. 12-HETE is a highly selective ligand used to label mu opioid receptors. 12-HETE can activate Cdc42, Rac1 and PI-3K, which then participate as upstream signalling molecµLes for PAK1 and JNK1 activation. 12-Hydroxyeicosatetraenoic acid (12-HETE) is a chemotactic stimµLus for epidermal cells. 12-hydroxyeicosatetraenoic acid (12-HETE), the main eicosanoid in skin, is assumed to have both pathophysiologic effects in inflammatory skin diseases such as psoriasis and atopic eczema and a physiologic role in the biology of cutaneous reparative processes. The modµLation of epidermal 12-HETE receptors by UV-B may partly explain the therapeutic effects of UV-B, but possibly also contribute to photodamage to skin. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).