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ELISA Perforin-1 (PRF1|PFP)

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Reactivity: (Homo sapiens) UniProt:P14222 Abbreviation:PRF1 Alternative Names:RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759|cytolysin|lymphocyte pore forming protein|perforin 1 Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.62 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.3% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Perforin is a cytolytic protein found in the granµLes of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granµLes of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranµLation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubµLes and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granµLes, and it is known to be a key effector molecµLe for T-cell- and natural killer-cell-mediated cytolysis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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