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ELISA Phosphatidylinositol-binding clathrin assembly protein (PICALM)

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Reactivity: (Homo sapiens) UniProt:Q13492 Abbreviation:PICALM Alternative Names:CALM; CLTH; LAP; clathrin assembly lymphoid-myeloid leukemia|phosphatidylinositol-binding clathrin assembly protein Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.118 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?7.5% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PICALM in samples. An antibody specific for PICALM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPICALM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PICALM is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PICALM bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dreyling et al. (1996) identified a breakpoint on chromosome 11 involving the previously unknown gene, CALM. CALM has a very high homology to the murine clathrin assembly protein ap3. Mao et al. (2001) determined the crystal structure of the N-terminal domain (the NAP domain) of the Drosophila Lap protein, which is homologous to PICALM. The structure revealed a novel fold consisting of a large double layer of sheets of 10 alpha helices and a unique site for binding phosphoinositides.This motif is found in other proteins predicted to have domains of similar structure (e.g., Huntingtin-interacting protein-1). The structure is in part similar to the epsin N-terminal (ENTH) domain, but epsin lacks the phosphatidylinositol-4,5-bisphosphate-binding site. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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