ELISA Peptidoglycan recognition protein L (PGRP-L)
Reactivity: (Homo sapiens)
UniProt:Q96PD5
Abbreviation:PGLYRP2
Alternative Names:HMFT0141; PGLYRPL; PGRP-L; PGRPL; TAGL-like; tagL; tagL-alpha; tagl-beta; peptidoglycan recognition protein L|peptidoglycan recognition protein-like
Application:ELISA
Range:0.156-10 ng/mL
Sensitivity:0.039 ng/mL
Intra-AssayCV:?4.7%
Inter-AssayCV:?6.9%
Recovery:0.9
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate PGLYRP2 in samples. An antibody specific for PGLYRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGLYRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PGLYRP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGLYRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 576-amino acid precursor protein contains an N-terminal signal peptide, followed by 2 transmembrane segments and an extracellµLar C terminus containing PGRP domains III, II, and I. PGRPL shares 40%, 33%, and 32% identity with PGRPS, PGRPI-alpha, and PGRPI-beta, respectively. RNA dot blot analysis detected strong PGRPL expression in adµLt liver and weak expression in fetal liver. Northern blot analysis detected 2.1- and 0.8-kb transcripts expressed in adµLt and fetal liver. PCR also detected low expression in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach, and testis. Transiently transfected COS-7 and embryonic kidney cells expressed PGRPL as a Triton X-100-soluble membrane protein with an apparent molecµLar mass of 65 kD.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).