ELISA Pepsin (PG)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:PG Alternative Names:N/A Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.81 pg/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.0% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PG in samples. An antibody specific for PG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PG is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PG bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Pepsin is an enzyme that whose precursor form (pepsinogen) is released by the chief cells in the stomach and that degrades food proteins into peptides. Pepsin was discovered in 1836 by Theodor Schwann who also coined this enzyme's name from the Greek word pepsis, meaning digestion (peptein: to digest). It was the first animal enzyme to be discovered, and, in 1929, it became one of the first enzymes to be crystallized, by John H. Northrop. Pepsin is a digestive protease.Pepsin functions best in acidic environments and is often found in an acidic environment, particµLarly those with a pH of 1.5 to 2. Pepsin denatures if the pH is more than 5.0.Pepsins shoµLd be stored at very cold temperatures (between ?20°C and ?80°C) to prevent autolysis (self-cleavage). Autolysis may also be prevented by storage of pepsins at pH 11 or by using modified pepsins (e.g., by reductive methylation). When the pH is adjusted back to 6.0 activity returns. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).