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ELISA Phosphatidylethanolamine-binding protein 1 (PEBP1)

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Reactivity: (Homo sapiens) UniProt:P30086 Abbreviation:PEBP1 Alternative Names:HCNP; PBP; PEBP; RKIP; Raf kinase inhibitory protein|hippocampal cholinergic neurostimµLating peptide|prostatic binding protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?9.6% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PEBP1 in samples. An antibody specific for PEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PEBP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:RKIP cDNA encodes a protein of 186 amino acids, the sequence of which is 95% identical to the bovine 21- to 23-kD protein. The rat protein shows 85.5% identity with the protein. highly expressed in the brain and associated with cytosolic proteins and small GTP-binding proteins. MµLtiple tissue Northern blots revealed the presence of a single mRNA in the different tissues of each species; a single band of 1.8 kb was identified in , 1.45 kb in mouse, and 1.2 kb in rat tissues. The mRNA is particµLarly highly expressed in rat and mouse testis, where the level was 30 times higher than that in the brain. PBP is expressed strongly in the cell bodies of oligodendrocytes but only weakly elsewhere. Schwann cells and spinal nerve roots showed intense cytoplasmic PBP immunoreactivity. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

1,220.00 € 1220.0 EUR 1,220.00 €

1,220.00 €

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