ELISA Pericentrin (PCNT)
Reactivity: (Homo sapiens)
UniProt:O95613
Abbreviation:PCNT
Alternative Names:KEN; MOPD2; PCN; PCNT2; PCNTB; PCTN2; SCKL4; kendrin|pericentrin B|pericentrin-2|pericentrin-380
Application:ELISA
Range:0.312-20 ng/mL
Sensitivity:0.119 ng/mL
Intra-AssayCV:?6.1%
Inter-AssayCV:?9..5%
Recovery:1.04
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate PCNT in samples. An antibody specific for PCNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PCNT is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCNT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Pericentrin (kendrin), also known as PCNT, is a protein binds to calmodµLin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubµLe nucleation component ?-tubµLin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell cycle progression.Expressed in all tissues tested, including placenta, liver, kidney and thymus. pericentrin is a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubµLe arrays.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).