ELISA Pericentriolar material 1 protein (PCM1)

Reactivity: (Homo sapiens) UniProt:Q15154 Abbreviation:PCM1 Alternative Names:PTC4; Application:ELISA Range:15.6-1000 pg/mL Sensitivity:3.9 pg/mL Intra-AssayCV:?5.6% Inter-AssayCV:?10.6% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PCM1 in samples. An antibody specific for PCM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PCM1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCM1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The PCM1 protein was originally identified by virtue of its distinct cell cycle-dependent association with the centrosome complex and microtubµLes. The protein appears to associate with the centrosome complex during the cell cycle. Dissociation occurs during mitosis when PCM1 is dispersed throµghout the cell. Immunolabeling studies performed found that PCM1 was present in centriolar satellites and in electron dense granµLes between 70 and 100 nm in diameter. These were originally thoµght to be scattered only around the centrosomes, but further studies proved that PCM1 was also found throµghout the cytoplasm.PCM1 forms a complex at the centrosome with DISC1 and BBS4, which provides a link between aberrant PCM1 and the abnormal cortical development associated with the pathology of schizophrenia. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).