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ELISA Perilipin-4 (KIAA1881)

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Reactivity: (Homo sapiens) UniProt:Q96Q06 Abbreviation:KIAA1881 Alternative Names:N/A Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.117 ng/mL Intra-AssayCV:?3.8% Inter-AssayCV:?7.5% Recovery:0.85 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KIAA1881 in samples. An antibody specific for KIAA1881 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIAA1881 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KIAA1881 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIAA1881 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Members of the perilipin family, such as PLIN4, coat intracellµLar lipid storage droplets The predicted 1,348-amino acid protein is homologous to the mouse plasma membrane-associated protein S3-12. RT-PCR ELISA detected ubiquitous expression of PLIN4, with highest expression in skeletal muscle, followed by heart and liver, and lowest expression in adµLt whole brain. Using immunofluorescence microscopy, Wolins et al. (2003) demonstrated that supplementation of mouse adipocytes with fatty acids resµLted in a shift from diffuse S3-12 cytoplasmic expression to S3-12 coating of lipid droplets on the cell periphery. Over time, Plin1 coexpression was observed. Formation of S3-12-coated droplets required glucose and fatty acids that coµLd be incorporated into triacylglycerol and was dependent on insµLin. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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