ELISA PXN (Ab-31) anti-

Quantity :100µL Clone Number: Aliases:FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_ antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P49023 Immunogen:Peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Paxillin. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC; WB:1:500-1:1000, IHC:1:50-1:200 Background: An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426 Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939 Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128 Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626 Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454 Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PXN Research Areas:Cancer;Signal transduction