ELISA Pan-AKT anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regµLatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regµLate a wide variety of cellµLar functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for fµLl activation of the AKT1 protein encoded by this gene. Immunogen: AKT1/AKT2/AKT3 Synonyms:AKT1/AKT2/AKT3 Observed MW:60 kDa Uniprot ID:P31749/P31751/Q9Y243 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC, IF, IP Recommended dilution:WB: 1:200-1:800; IHC: 1:20-1:100; IF: 1:20-1:100; IP: 1:20-1:100 Gene ID: Research Area: