ELISA Pfu DNA Polymerase

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions resµLts in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis. Components: Pfu DNA polymerase (2.5 U/µL or 5 U/µL-please see user manual), 10x Pfu buffer Features 3'-5' exonuclease activity provides a low error rate One of the most thermostable DNA polymerases known Lack of extendase activity means no unwanted 3’ overhangs Optimal for blunt-ended PCR cloning Optimum temperature near 75°C 95% active after 1hour incubation at 98°C Applications High-fidelity PCR and primer-extension reactions PCR cloning and blunt-ended amplification product generation Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate. Store all components at –20°C