ELISA Phi29 DNA Polymerase

Product Details This product is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the φ29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'→5' proofreading exonuclease activity to ensure high fidelity of resµLting DNA. Its properties of mµLtiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circµLar DNA. Highlights · Isothermal amplification; · High fidelity; high efficiency; high sensitivity; high yield; · Random primers or specific N9 primers can be used. Application Sequencing, SNP genotyping, STR/ microsatellite analysis, virus detection, miRNA detection, etc. Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component LP101-01 LP101-02 Phi29 DNA Polymerase 250 units 5×250 units 10×Phi29 DNA Polymerase Buffer 50 μl 250 μl Phi29 Random Primers 125 μl 625 μl 10 mM dNTPs 25 μl 125 μl 10×DNA Loading Buffer 1 mL 1 mL