ELISA Phi29 DNA Polymerase
Product Details
This product is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the φ29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'→5' proofreading exonuclease activity to ensure high fidelity of resµLting DNA. Its properties of mµLtiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circµLar DNA.
Highlights
· Isothermal amplification;
· High fidelity; high efficiency; high sensitivity; high yield;
· Random primers or specific N9 primers can be used.
Application
Sequencing, SNP genotyping, STR/ microsatellite analysis, virus detection, miRNA detection, etc.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
Component LP101-01 LP101-02
Phi29 DNA Polymerase 250 units 5×250 units
10×Phi29 DNA Polymerase Buffer 50 μl 250 μl
Phi29 Random Primers 125 μl 625 μl
10 mM dNTPs 25 μl 125 μl
10×DNA Loading Buffer 1 mL 1 mL